Laura Lagostena

Research staff

Education / Training:
01/1999 - 04/2002: PhD in Neuroscience “Calcium Dynamics in Sensory and Supporting Cells of the Organ of Corti”, Open University, London, United Kingdom
09/1984 - 05/1991Degree in biology “Neurotoxic effect of a phospholipase isolated from snake venom on ion currents in cultured neurons”, University of Genova (Italy)

Job Positions:
12/2018 - Present: Researcher (permanent position) at Istituto di Biofisica - CNR
10/2016 - 12/2018: Post Doc at Fondazione Telethon - Istituto di Biofisica
09/2013 - 09/2016: Research Fellow at Istituto di Biofisica - CNR
09/2012 - 08/2013: Post Doc at Istituto di Tecnologia (IIT)
12/2008 - 12/2010: Research Fellow at Dipartimento di Scienze Biomediche - Università di Modena
10/2005 - 12/2008: Research Fellow at Settore di Neurobiologia - SISSA
05/2003 - 12/2004: Post Doc at Istituto Dermopatico dell'Immacolata (IDI)
10/1998 - 05/2003: Research Fellow at Scuola Internazionale degli Studi Superiori Applicati (SISSA)
12/1995 - 08/1998: Scholarship at Istituto di Cibernetica e Biofisica - CNR
06/1991 - 07/1994: Scholarship at Ospedale Civile di Sondrio

orcid://0000-0001-6440-3720,

Contact:

- Email laura.lagostena@ibf.cnr.it
- Phone +39-010-6475560
- Location Genova

Research interests:

- Neurotoxic effect of per- and polyfluoroalkyl substances (PFAS) (Principal investigator)
- Recordings of electronic currents from plant cytochromes
- Functional and structural studies of human CLC protein complexes involved in genetic diseases

Key publications:

Di Zanni E, Palagano E, Lagostena L, Strina D, Rehman A, Abinun M, De Somer L, Martire B, Brown J, Kariminejad A, Balasubramaniam S, Baynam G, Gurrieri F, Pisanti MA, De Maggio I, Abboud MR, Chiesa R, Burren CP, Villa A, Sobacchi C, Picollo A, . "Pathobiologic Mechanisms of Neurodegeneration in Osteopetrosis Derived From Structural and Functional Analysis of 14 ClC-7 Mutants". Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research. 2021 36(3):531-545

Badurek S, Griguoli M, Asif-Malik A, Zonta B, Guo F, Middei S, Lagostena L, Jurado-Parras MT, Gillingwater TH, Gruart A, Delgado-García JM, Cherubini E, Minichiello L, "Immature Dentate granule Cells Require Ntrk2/Trkb for the Formation of Functional Hippocampal Circuitry". iScience. 2020 23 (5).

Lagostena L, Zifarelli G, Picollo A, "New Insights into the Mechanism of NO3 - Selectivity in the Human Kidney Chloride Channel ClC-Ka and the CLC Protein Family". Journal of the American Society of Nephrology : JASN. 2019 30 (2).

Lagostena L, Rosato-Siri M, D'Onofrio M, Brandi R, Arisi I, Capsoni S, Franzot J, Cattaneo A, Cherubini E, "In the adult hippocampus, chronic nerve growth factor deprivation shifts GABAergic signaling from the hyperpolarizing to the depolarizing direction". The Journal of neuroscience : the official journal of the Society for Neuroscience. 2010 30 (3).

Lagostena L, Danober L, Challal S, Lestage P, Mocaër E, Trocmé-Thibierge C, Cherubini E, "Modulatory effects of S 38232, a non alpha-7 containing nicotine acetylcholine receptor agonist on network activity in the mouse hippocampus". NEUROPHARMACOLOGY. 2010 58 (4-5).

Publications (13):

Immature Dentate Granule Cells Require Ntrk2/Trkb for the Formation of Functional Hippocampal Circuitry
Badurek, Sylvia; Griguoli, Marilena; Asif-Malik, Aman; Zonta, Barbara; Guo, Fei; Middei, Silvia; Lagostena, Laura; Teresa Jurado-Parras, Maria; Gillingwater, Thomas H.; Gruart, Agnes; Maria Delgado-Garcia, Jose; Cherubini, Enrico; Minichiello, Liliana
Iscience, 23, 2020
DOI: 10.1016/j.isci.2020.101078

Pathobiologic Mechanisms of Neurodegeneration in Osteopetrosis Derived from Structural and Functional Analysis of 14 ClC-7 Mutants
Eleonora Di Zanni, Eleonora Palagano, Laura Lagostena, Dario Strina, Asma Rehman, Mario Abinun, Lien De Somer, Baldassarre Martire, Justin Brown, Ariana Kariminejad, Shanti Balasubramanian, Gareth Baynam, Fiorella Gurrieri, Maria A Pisanti,...
Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research, 2020
DOI: 10.1002/jbmr.4200

The mechanism and energetics of a ligand-controlled hydrophobic gate in a mammalian two pore channel
Milenkovic, Stefan; Bodrenko, Igor, V; Lagostena, Laura; Gradogna, Antonella; Serra, Giovanni; Bosin, Andrea; Carpaneto, Armando; Ceccarelli, Matteo
Physical Chemistry Chemical Physics, 22, 2020
DOI: 10.1039/d0cp00805b

A perspective on the modulation of plant and animal two pore channels (TPCs) by the flavonoid naringenin
Benkerrou, D.; Minicozzi, V.; Gradogna, A.; Milenkovic, S.; Bodrenko, I. V.; Festa, M.; Lagostena, L.; Cornara, L.; D'Amore, A.; Ceccarelli, M.; Filippini, A.; Carpaneto, A.
biophysical chemistry (print), 254, 2019
DOI: 10.1016/j.bpc.2019.106246

New Insights into the Mechanism of NO3 - Selectivity in the Human Kidney Chloride Channel ClC-Ka and the CLC Protein Family
Lagostena L, Zifarelli G, Picolllo A
Journal of the American Society of Nephrology, 30, 2019
DOI: 10.1681/ASN.2018060593

Electron current recordings in living cells
Trost P, Picco C, Scholz-Starke J, Festa M, Lagostena L, Costa A, Sparla F, Carpaneto A
Biophysical chemistry , 229, 2017
DOI: 10.1016/j.bpc.2017.05.006

Naringenin Impairs Two-Pore Channel 2 Activity And Inhibits VEGF-Induced Angiogenesis
Pafumi I, Festa M, Papacci F, Lagostena L, Giunta C, Gutla V, Cornara L, Favia A, Palombi F, Gambale F, Filippini A, Carpaneto A
Scientific reports , 7, 2017
DOI: 10.1038/s41598-017-04974-1

The human two-pore channel 1 is modulated by cytosolic and luminal calcium
Lagostena L, Festa M, Pusch M, Carpaneto A
Scientific reports , 7, 2017
DOI: 10.1038/srep43900

The signaling lipid phosphatidylinositol-3,5-bisphosphate targets plant CLC-a anion/H exchange activity.
Carpaneto A, Boccaccio A, Lagostena L, Di Zanni E, Scholz-Starke J
EMBO reports, 18, 2017
DOI: 10.15252/embr.201643814

Identification and Functional Characterization of CLCN1 Mutations Found in Nondystrophic Myotonia Patients
Vindas-Smith R.; Fiore M.; Vasquez M.; Cuenca P.; del Valle G.; Lagostena L.; Gaitan-Penas H.; Estevez R.; Pusch M.; Morales F.
Human mutation, 37, 2016
DOI: 10.1002/humu.22916

Using the plant vacuole as a biological system to investigate the functional properties of exogenous channels and transporters
M. Festa , L. Lagostena , A. Carpaneto
Biochimica et biophysica acta. Biomembranes, 1858, 2016
DOI: 10.1016/j.bbamem.2015.09.022

GlialCAM, a CLC-2 Cl- Channel Subunit, Activates the Slow Gate of CLC Chloride Channels
Jeworutzki, Elena; Lagostena, Laura; Elorza-Vidal, Xabier; Lopez-Hernandez, Tania; Estevez, Raul; Pusch, Michael
Biophysical journal, 107, 2014
DOI: 10.1016/j.bpj.2014.07.040

Large-conductance calcium-activated anion channel characteristics in neuroblastoma cells
Nobile, M.; Lagostena, L.
GENERAL PHYSIOLOGY AND BIOPHYSICS, 19, 2000