Personal Card



Samuele Raccosta

Research staff

2011: PhD in Physics (6 months spent in Copenhagen at Prof. Vestergaard’s lab for studies on aSN fibrillation) 2011-2012: fellowship at Biophysics and Nanoscience Centre (Prof. Cannistraro’s lab) 2014: 1 month spent at AppNano for studies on Scanning Thermal Microscopy (Mountain View, California) Since 2014 I work at Institute of Biophysics and my research activity mainly concerns biophysical studies on extracellular vesicles (mechanical properties, composition, functionalization), protein aggregation and protein-protein interaction. Expertise: AFM, Light Scattering, Differential Scanning Calorimetry, Optical spectroscopy, Circular Dichroism. Actually, I’m working on two Horizon2020 projects: Ves4Us (FET-open call) and BOW (FET proactive)

orcid://0000-0001-7775-5928,

Contact:

- Email samuele.raccosta@ibf.cnr.it
- Phone +39-091-6809311
- Location Palermo

Research interests:

- Molecular bases of serpinopathies
- Characterisation of extracellular vescicles
- Laboratory for Advanced and Scattering Technologies (LAST)
- Model Membrane Systems and Interaction Studies
- Cell-based technologies and bioactives for research and innovation (Cell-Tech Hub)

Key publications:

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae. Adamo et al. Journal of Extracellular Vesicles, 2021, 10(6), e12081

Palmitoylation is a post-translational modification of Alix regulating the membrane organization of exosome-like small extracellular vesicles. Romancino et al. Biochimica et Biophysica Acta - General Subjects, 2018, 1862(12), pp. 2879–2887

Scaling concepts in serpin polymer physics. Raccosta et al. Materials, 2021, 14(10), 2577

Conductive atomic force microscopy study of single molecule electron transport through the Azurin-gold nanoparticle system. Raccosta et al. Applied Physics Letters, 2013, 102(20), 203704

Thermodynamic versus conformational metastability in fibril-forming lysozyme solutions. Raccosta et al. Journal of Physical Chemistry B, 2012, 116(40), pp. 12078–12087

Publications (25):

DetectEV: A functional enzymatic assay to assess integrity and bioactivity of extracellular vesicles
Adamo, Giorgia; Picciotto, Sabrina; Gargano, Paola; Paterna, Angela; Raccosta, Samuele; Rao, Estella; Romancino, Daniele Paolo; Ghersi, Giulio; Manno, Mauro; Salamone, Monica; Bongiovanni, Antonella
JOURNAL OF EXTRACELLULAR VESICLES, 14, 2025
DOI: 10.1002/jev2.70030

A decision-making tool to navigate through Extracellular Vesicle research and product development
Loria, Francesca; Picciotto, Sabrina; Adamo, Giorgia; Zendrini, Andrea; Raccosta, Samuele; Manno, Mauro; Bergese, Paolo; Liguori, Giovanna L.; Bongiovanni, Antonella; Zarovni, Nataša
JOURNAL OF EXTRACELLULAR VESICLES, 2024
DOI: 10.1101/2023.11.16.567368

Extracellular vesicles from the microalga Tetraselmis chuii are biocompatible and exhibit unique bone tropism along with antioxidant and anti-inflammatory properties
Adamo, Giorgia; Santonicola, Pamela; Picciotto, Sabrina; Gargano, Paola; Nicosia, Aldo; Longo, Valeria; Aloi, Noemi; Romancino, Daniele; Paterna, Angela; Rao, Estella; Raccosta, Samuele; Noto, Rosina; Salamone, Monica; Deidda, Irene; Costa, Salvatore; DI SANO, Caterina; Zampi, Giuseppina; Morsbach, Svenja; Landfester, Katharina; Colombo, Paolo; Wei, Mingxing; Bergese, Paolo; Touzet, Nicolas; Manno, Mauro; DI SCHIAVI, Elia; Bongiovanni, Antonella
COMMUNICATIONS BIOLOGY, 7, 2024
DOI: 10.1038/s42003-024-06612-9

alphaS1-Casein administration to a C. elegans model of Alzheimer's disease through proteoliposome delivery
Carrotta, Rita; Paterna, Angela; Santonicola, Pamela; DI PRIMA, Giulia; Rao, Estella; Raccosta, Samuele; Zampi, Giuseppina; Oscar, Moran; Manno, Mauro; DI SCHIAVI, Elia; Librizzi, Fabio
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 52, 2023

αS1-Casein-Loaded Proteo-liposomes as Potential Inhibitors in Amyloid Fibrillogenesis: In Vivo Effects on a C. elegans Model of Alzheimer’s Disease
Paterna, A.; Santonicola, P.; Di Prima, G.; Rao, E.; Raccosta, S.; Zampi, G.; Russo, C.; Moran, O.; Manno, M.; Di Schiavi, E.; Librizzi, F.; Carrotta, R.
ACS CHEMICAL NEUROSCIENCE, 14, 2023
DOI: 10.1021/acschemneuro.3c00239

Extracellular Vesicles From Microalgae: Uptake Studies in Human Cells and Caenorhabditis elegans
Picciotto, Sabrina; Santonicola, Pamela; Paterna, Angela; Rao, Estella; Raccosta, Samuele; Romancino Daniele, Paolo; Noto, Rosina; Touzet, Nicolas; Manno, Mauro; DI SCHIAVI, Elia; Bongiovanni, Antonella; Adamo, Giorgia
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 10, 2022
DOI: 10.3389/fbioe.2022.830189

Isolation of extracellular vesicles from microalgae: Towards the production of sustainable and natural nanocarriers of bioactive compounds
Picciotto, Sabrina; Barone, Maria E.; Fierli, David; Aranyos, Anita; Adamo, Giorgia; Bo?i?, Darja; Romancino, Daniele P.; Stanly, Christopher; Parkes, Rachel; Morsbach, Svenja; Raccosta, Samuele; Paganini, Carolina; Cusimano, Antonella; Martorana, Vincenzo; Noto, Rosina; Carrotta, Rita; Librizzi, Fabio; Capasso Palmiero, Umberto; Santonicola, Pamela; Igli?, Ales; Gai, Meiyu; Corcuera, Laura; Kisslinger, Annamaria; Di Schiavi, Elia; Landfester, Katharina; Liguori, Giovanna L.; Kralj-Igli?, Veronika; Arosio, Paolo; Pocsfalvi, Gabriella; Manno, Mauro; Touzet, Nicolas; Bongiovanni, Antonella
biomaterials science (print)issn: 2047-4830royal society of chemistry, 9, 2021
DOI: 10.1039/d0bm01696a

Nanoalgosomes: Introducing extracellular vesicles produced by microalgae
Adamo, Giorgia; Fierli, David; Romancino, Daniele P.; Picciotto, Sabrina; Barone, Maria E.; Aranyos, Anita; Bo?i?, Darja; Morsbach, Svenja; Raccosta, Samuele; Stanly, Christopher; Paganini, Carolina; Gai, Meiyu; Cusimano, Antonella; Martorana, Vincenzo; Noto, Rosina; Carrotta, Rita; Librizzi, Fabio; Randazzo, Loredana; Parkes, Rachel; Capasso Palmiero, Umberto; Rao, Estella; Paterna, Angela; Santonicola, Pamela; Igli?, Ales; Corcuera, Laura; Kisslinger, Annamaria; Di Schiavi, Elia; Liguori, Giovanna L.; Landfester, Katharina; Kralj-Igli?, Veronika; Arosio, Paolo; Pocsfalvi, Gabriella; Touzet, Nicolas; Manno, Mauro; Bongiovanni, Antonella
journal of extracellular vesiclesissn: 2001-3078järfälla : co-action pub., 10, 2021
DOI: 10.1002/jev2.12081

Preliminary results of citraves(TM) effects on low density lipoprotein cholesterol and waist circumference in healthy subjects after 12 weeks: A pilot open-label study
Raimondo, S. and Nikolic, D. and Conigliaro, A. and Giavaresi, G. and Sasso, B.L. and Giglio, R.V. and Chianetta, R. and Manno, M. and Raccosta, S. and Corleone, V. and Ferrante, G. and Citarrella, R. and Rizzo, M. and De Leo, G. and Ciaccio, M. and Montalto, G. and Alessandro, R.
metabolitesissn: 2218-1989molecular diversity preservation international, 11, 2021
DOI: 10.3390/metabo11050276

Scaling concepts in serpin polymer physics
Raccosta S.; Librizzi F.; Jagger A.M.; Noto R.; Martorana V.; Lomas D.A.; Irving J.A.; Manno M.
materials (basel)issn: 1996-1944molecular diversity preservation international, 14, 2021
DOI: 10.3390/ma14102577

Osteosarcoma cell-derived exosomes affect tumor microenvironment by specific packaging of microRNAs
Raimondi, L., De Luca, A., Gallo, A., Costa, V., Russelli, G., Cuscino, N., Manno, M., Raccosta, S., Carina, V., Bellavia, D., Conigliaro, A., Alessandro, R., Fini, M., Conaldi, P.G., Giavaresi, G.
Carcinogenesis, 41, 2020
DOI: 10.1093/carcin/bgz130

Amyloid jams: Mechanical and dynamical properties of an amyloid fibrillar network
Martorana V, Raccosta S, Giacomazza D, Ditta LA, Noto R, Biagio PLS, Manno M
Biophysical chemistry, 253, 2019
DOI: 10.1016/j.bpc.2019.106231

Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesis
Raimondo S, Saieva L, Vicario E, Pucci M, Toscani D, Manno M, Raccosta S, Giuliani N, Alessandro R
Journal of hematology & oncology, 12, 2019
DOI: 10.1186/s13045-018-0689-y

Palmitoylation is a post -translational modification of Alix regulating the membrane organization of exosome-like small extracellular vesicles
Romancino, Daniele P.; Buffa, Valentina; Caruso, Stefano; Ferrara, Ines; Raccosta, Samuele; Notaro, Antonietta; Campos, Yvan; Noto, Rosina; Martorana, Vincenzo; Cupane, Antonio; Giallongo, Agata; d'Azzo, Alessandra; Manno, Mauro; Bongiovanni, Antonella
biochimica et biophysica acta. g, general subjects (print), 1862, 2018
DOI: 10.1016/j.bbagen.2018.09.004

Interleukin 3-receptor targeted exosomes inhibit in vitro and in vivo Chronic Myelogenous Leukemia cell growth
Bellavia D, Raimondo S, Calabrese G, Forte S, Cristaldi M, Patinella A, Memeo L, Manno M, Raccosta S, Diana P, Cirrincione G, Giavaresi G, Monteleone F, Fontana S, De Leo G, Alessandro R
Theranostics, 7, 2017
DOI: 10.7150/thno.17092

Embelin binds to human neuroserpin and impairs its polymerisation
Saga, Giorgia; Sessa, Fabio; Barbiroli, Alberto; Santambrogio, Carlo; Russo, Rosaria; Sala, Michela; Raccosta, Samuele; Martorana, Vincenzo; Caccia, Sonia; Noto, Rosina; Moriconi, Claudia; Miranda, Elena; Grandori, Rita; Manno, Mauro; Bolognesi, Martino;
Scientific reports , 6, 2016
DOI: 10.1038/srep18769

CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA
Conigliaro A.; Costa V.; Lo Dico A.; Saieva L.; Buccheri S.; Dieli F.; Manno M.; Raccosta S.; Mancone C.; Tripodi M.; De Leo G.; Alessandro R.
Molecular cancer , 14, 2015
DOI: 10.1186/s12943-015-0426-x

Curcumin inhibits in vitro and in vivo chronic myelogenous leukemia cells growth: A possible role for exosomal disposal of miR-21
Taverna S.; Giallombardo M.; Pucci M.; Flugy A.; Manno M.; Raccosta S.; Rolfo C.; De Leo G.; Alessandro R.
Oncotarget , 6, 2015
DOI: 10.18632/oncotarget.4204

Involvement of multiple myeloma cell-derived exosomes in osteoclast differentiation
Raimondi L.; De Luca A.; Amodio N.; Manno M.; Raccosta S.; Taverna S.; Bellavia D.; Naselli F.; Fontana S.; Schillaci O.; Giardino R.; Fini M.; Tassone P.; Santoro A.; De Leo G.; Giavaresi G.; Alessandro R.
Oncotarget , 6, 2015
DOI: 10.18632/oncotarget.3830

The stability and activity of human neuroserpin are modulated by a salt bridge that stabilises the reactive centre loop
Noto R.; Randazzo L.; Raccosta S.; Caccia S.; Moriconi C.; Miranda E.; Martorana V.; Manno M.
Scientific reports , 5, 2015
DOI: 10.1038/srep13666

Custom system for single molecule force spectroscopy
Landini, E; Raccosta, S; D'Anca, F; Zora, M; Mazzola, S; Sanbiagio, Pl; Martorana, V; Vassalli, M
MICROSCOPIE, 11, 2014

Wildtype and A30P Mutant Alpha-Synuclein Form Different Fibril Structures
Nielsen SB, Macchi F, Raccosta S, Langkilde AE, Giehm L, Kyrsting A, Pii Svane AS, Manno M, Christiansen G, Nielsen NC, Oddershede L, Vestergaard B, Otzen DE
PloS one, 8, 2013
DOI: 10.1371/journal.pone.0067713

Thermodynamic versus Conformational Metastability in Fibril-Forming Lysozyme Solutions
Raccosta S; Martorana V; Manno M
Journal of physical chemistry b, 116, 2012

Existence of metastable intermediate lysozyme conformation highlights the role of alcohols in altering protein stability
D'Amico M, Raccosta S, Cannas M, Martorana V, Manno M
Journal of physical chemistry b, 115, 2011

Irreversible gelation of thermally unfolded lysozyme: structural and mechanical properties of lysozyme aggregates
Raccosta S, Manno M, Bulone D, Giacomazza D, Militello V, Martorana V, San Biagio PL
European biophysics journal with biophysics letters, 39, 2010
DOI: 10.1007/s00249-009-0503-4