Ferrara, Silvia; Braggiotti, Bianca; Mastrangelo, Eloise; Di Gennaro, Patrizia; Bertoni, Giovanni; Milani, Mario
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 747, 2025
DOI: 10.1016/j.bbrc.2024.151281
4-(3-Phenylsulfonylindol-2-yl)-1-(pyridin-2-yl)piperazinyl-methanones as Potent Inhibitors of both SARS-CoV-2 and HCoV-OC43 Viruses
Puxeddu, M.; Donalisio, M.; Bugert, J. J.; Corona, A.; Cocomazzi, P.; MILANI DE MAYO DE MARI, Mario; Hucke, F.; Arduino, I.; Esposito, F.; Moretti, P.; Ortore, M. G.; Nalli, M.; Manetto, S.; Mazzoccanti, G.; Bigogno, C.; Dondio, G.; Scio, P.; Coluccia, A.; Fracella, M.; Antonelli, G.; Lembo, D.; Tramontano, E.; Silvestri, R.; Mastrangelo, E.; La Regina, G.
ACS INFECTIOUS DISEASES, 10, 2024
DOI: 10.1021/acsinfecdis.4c00108
CHCHD4 binding affects the active site of apoptosis inducing factor (AIF): Structural determinants for allosteric regulation
Fagnani, E.; Cocomazzi, P.; Pellegrino, S.; Tedeschi, G.; Scalvini, F. G.; Cossu, F.; Da Vela, S.; Aliverti, A.; Mastrangelo, E.; Milani, M.
STRUCTURE, 2024
DOI: 10.1016/j.str.2024.02.008
Stabilization of the retromer complex: Analysis of novel binding sites of bis-1,3-phenyl guanylhydrazone 2a to the VPS29/VPS35 interface
Fagnani, E.; Bonì, F.; Seneci, P.; Gornati, D.; Muzio, L.; Mastrangelo, E.; MILANI DE MAYO DE MARI, Mario
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 23, 2024
DOI: 10.1016/j.csbj.2024.02.026
Studying an orphan disease by a biophysical approach: The case of cblC
Vilasi, S.; Longo, L.; Randazzo, L.; Bollati, M.; Carrotta, R.; Costa, M. A.; De Rosa, M.; Mangione, M. R.; Martorana, V.; Milani, M.; Ortore, M. G.; Tutone, M.; Passantino, R.
IL NUOVO CIMENTO C, 47, 2024
DOI: 10.1393/ncc/i2024-24318-2
Virucidal Activity of the Pyridobenzothiazolone Derivative HeE1-17Y against Enveloped RNA Viruses
Bonotto R.M.; Boni F.; Milani M.; Chaves-Sanjuan A.; Franze S.; Selmin F.; Felicetti T.; Bolognesi M.; Konstantinidou S.; Poggianella M.; Marquez C.L.; Dattola F.; Zoppe M.; Manfroni G.; Mastrangelo E.; Marcello A.
viruses, 14, 2022
DOI: 10.3390/v14061157
A novel hotspot of gelsolin instability triggers an alternative mechanism of amyloid aggregation
Bollati, Michela; Diomede, Luisa; Giorgino, Toni; Natale, Carmina; Fagnani, Elisa; Boniardi, Irene; Barbiroli, Alberto; Alemani, Rebecca; Beeg, Marten; Gobbi, Marco; Fakin, Ana; Mastrangelo, Eloise; Milani, Mario; Presciuttini, Gianluca; Gabellieri, Edi; Cioni, Patrizia; de Rosa, Matteo
computational and structural biotechnology journalissn: 2001-0370chalmers university of technology,, 19, 2021
DOI: 10.1016/j.csbj.2021.11.025
Combined in silico and in vitro approaches identified the antipsychotic drug lurasidone and the antiviral drug elbasvir as SARS-CoV2 and HCoV-OC43 inhibitors
Milani M.; Donalisio M.; Bonotto R.M.; Schneider E.; Arduino I.; Boni F.; Lembo D.; Marcello A.; Mastrangelo E.
antiviral research (print)issn: 0166-3542elsevier/north-holland,, 189, 2021
DOI: 10.1016/j.antiviral.2021.105055
Structure-based identification of a new IAP-targeting compound that induces cancer cell death inducing NF-kappa B pathway
Cossu, Federica; Camelliti, Simone; Lecis, Daniele; Sorrentino, Luca; Majorini, Maria Teresa; Milani, Mario; Mastrangelo, Eloise
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 19, 2021
DOI: 10.1016/j.csbj.2021.11.034
Synthesis and Characterization of Novel Mono- And Bis-Guanyl Hydrazones as Potent and Selective ASIC1 Inhibitors Able to Reduce Brain Ischemic Insult
Gornati D.; Ciccone R.; Vinciguerra A.; Ippati S.; Pannaccione A.; Petrozziello T.; Pizzi E.; Hassan A.; Colombo E.; Barbini S.; Milani M.; Caccavone C.; Randazzo P.; Muzio L.; Annunziato L.; Menegon A.; Secondo A.; Mastrangelo E.; Pignataro G.; Seneci P.
journal of medicinal chemistry (online), 64, 2021
DOI: 10.1021/acs.jmedchem.1c00305
Computational and Experimental Characterization of NF023, A Candidate Anticancer Compound Inhibiting cIAP2/TRAF2 Assembly
Federica Cossu, Luca Sorrentino, Elisa Fagnani, Mattia Zaffaroni, Mario Milani, Toni Giorgino, Eloise Mastrangelo
Journal of chemical information and modeling, 60, 2020
DOI: 10.1021/acs.jcim.0c00518
Missense mutations affecting Ca2+-coordination in GCAP1 lead to cone-rod dystrophies by altering protein structural and functional properties
Dal Cortivo, Giuditta; Marino, Valerio; Boni, Francesco; Milani, Mario; Dell'Orco, Daniele
Biochimica Et Biophysica Acta-molecular Cell Research, 1867, 2020
DOI: 10.1016/j.bbamcr.2020.118794
Modulation of guanylate cyclase activating protein 1 (Gcap1) dimeric assembly by ca2+ or mg2+: Hints to understand protein activity
Bonì, F., Marino, V., Bidoia, C., Mastrangelo, E., Barbiroli, A., Dell’Orco, D., Milani, M.
Biomolecules, 10, 2020
DOI: 10.3390/biom10101408
Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis
Muzio, Luca; Sirtori, Riccardo; Gornati, Davide; Eleuteri, Simona; Fossaghi, Andrea; Brancaccio, Diego; Manzoni, Leonardo; Ottoboni, Linda; De Feo, Luca; Quattrini, Angelo; Mastrangelo, Eloise; Sorrentino, Luca; Scalone, Emanuele; Comi, Giancarlo; Marinel
Nature Communications, 11, 2020
DOI: 10.1038/s41467-020-17524-7
The structure of N184K amyloidogenic variant of gelsolin highlights the role of the H-bond network for protein stability and aggregation properties
Matteo de Rosa, Alberto Barbiroli, Francesco Bonì, Emanuele Scalone, Davide Mattioni, Maria A Vanoni, Marco Patrone, Michela Bollati, Eloise Mastrangelo, Toni Giorgino, Mario Milani
European biophysics journal, 49, 2020
DOI: 10.1007/s00249-019-01409-9
An alternative non-proteolytic mechanism may underlie AGel amyloidosis
Bonì F, Milani M, Mastrangelo E, Babiroli A, Diomede L, de Rosa M
Amyloid: the international journal of experimental and clinical investigation: the official journal of the International Society of Amyloidosis, 26, 2019
DOI: 10.1080/13506129.2019.1582510
Broad spectrum anti-flavivirus pyridobenzothiazolones leading to less infective virions
Cannalire R, Tarantino D, Piorkowski G, Carletti T, Massari S, Felicetti T, Barreca ML, Sabatini S, Tabarrini O, Marcello A, Milani M, Cecchetti V, Mastrangelo E, Manfroni G, Querat G
Antiviral research, 167, 2019
DOI: 10.1016/j.antiviral.2019.03.004
High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion
Bollati M, Scalone E, Bonì F, Mastrangelo E, Giorgino T, Milani M, de Rosa M
Biochemical and biophysical research communications, 518, 2019
DOI: 10.1016/j.bbrc.2019.08.013
Nanobody interaction unveils structure, dynamics and proteotoxicity of the Finnish-type amyloidogenic gelsolin variant
Giorgino T, Mattioni D, Hassan A, Milani M, Mastrangelo E, Barbiroli A, Verhelle A, Gettemans J, Barzago MM, Diomede L, de Rosa M
Biochimica et biophysica acta. Molecular basis of disease, 1865, 2019
DOI: 10.1016/j.bbadis.2019.01.010
Structural and functional characterization of TgpA, a critical protein for the viability of Pseudomonas aeruginosa
Uruburu M, Mastrangelo E, Bolognesi M, Ferrara S, Bertoni G, Milani M
Journal of structural biology, 205, 2019
DOI: 10.1016/j.jsb.2018.12.004
Structure-Activity Relationship of NF023 Derivatives Binding to XIAP-BIR1
Sorrentino L, Cossu F, Milani M, Malkoc B, Huang WC, Tsay SC, Ru Hwu J, Mastrangelo E
ChemistryOpen, 8, 2019
DOI: 10.1002/open.201900059
Targeting the BIR Domains of Inhibitor of Apoptosis (IAP) Proteins in Cancer Treatment
Cossu F, Milani M, Mastrangelo E, Lecis D
Computational and Structural Biotechnology Journal, 17, 2019
DOI: 10.1016/j.csbj.2019.01.009
The structure of N184K amyloidogenic variant of gelsolin highlights the role of the H-bond network for protein stability and aggregation properties
de Rosa M, Barbiroli A, Bonì F, Scalone E, Mattioni D, Vanoni MA, Patrone M, Bollati M, Mastrangelo E, Giorgino T, Milani M
European biophysics journal, 49, 2019
DOI: 10.1007/s00249-019-01409-9
A stereospecific carboxyl esterase from Bacillus coagulans hosting nonlipase activity within a lipase-like fold
De Vitis V, Nakhnoukh, Pinto A, Contente ML, Barbiroli A, Milani M, Bolognesi M, Molinari F, Gourlay LJ, Romano D
The FEBS journal (online), 285, 2018
DOI: 10.1111/febs.14368
Functionalized 2,1-benzothiazine 2,2-dioxides as new inhibitors of Dengue NS5 RNA-dependent RNA polymerase
Cannalire R, Tarantino D, Astolfi A, Barreca ML, Sabatini S, Massari S, Tabarrini O, Milani M, Querat G, Mastrangelo E, Manfroni G, Cecchetti V
European journal of medicinal chemistry (online), 143, 2018
DOI: 10.1016/j.ejmech.2017.10.064
Gelsolin pathogenic Gly167Arg mutation promotes domain-swap dimerization of the protein
Bonì F, Milani M, Barbiroli A, Diomede L, Mastrangelo E, de Rosa M
Human molecular genetics (online), 27, 2018
DOI: 10.1093/hmg/ddx383
Glycine Amidinotransferase (GATM), Renal Fanconi Syndrome, and Kidney Failure
Reichold M, Klootwijk ED, Reinders J, Otto EA, Milani M, Broeker C, Laing C, Wiesner J, Devi S, Zhou W, Schmitt R, Tegtmeier I, Sterner C, Doellerer H, Renner K, Oefner PJ, Dettmer K, Simbuerger JM, Witzgall R, Stanescu HC, Dumitriu S, Iancu D, Patel V, M
Journal of the American Society of Nephrology, 29, 2018
DOI: 10.1681/ASN.2017111179
Identification of a small molecule that compromises the structural integrity of viroplasms and rotavirus double-layered particles
Eichwald C, De Lorenzo G, Schraner EM, Papa G, Bollati M, Swuec P, de Rosa M, Milani M, Mastrangelo E, Ackermann M, Burrone OR, Arnoldi F
Journal of virology, 92, 2018
DOI: 10.1128/JVI.01943-17
Role and inhibition of GLI1 protein in cancer
Mastrangelo E, Milani M
Lung cancer , 9, 2018
DOI: 10.2147/LCTT.S124483
Structure-based design and molecular profiling of Smac-mimetics selective for cellular IAPs
Corti A, Milani M, Lecis D, Seneci P, de Rosa M, Mastrangelo E, Cossu F
The FEBS journal, 285, 2018
DOI: 10.1111/febs.14616
Structural bases of the altered catalytic properties of a pathogenic variant of apoptosis inducing factor
Sorrentino L, Cossu F, Milani M, Aliverti A, Mastrangelo E
Biochemical and biophysical research communications, 490, 2017
DOI: 10.1016/j.bbrc.2017.06.156
Crystal structure of YeaZ from Pseudomonas aeruginosa
Vecchietti D, Ferrara S, Rusmini R, Macchi R, Milani M, Bertoni G
Biochemical and biophysical research communications, 470, 2016
DOI: 10.1016/j.bbrc.2016.01.008
Molecular basis of a novel renal amyloidosis due to N184K gelsolin variant
Bonì F, Milani M, Porcari R, Barbiroli A, Ricagno S, de Rosa M
Scientific reports , 6, 2016
DOI: 10.1038/srep33463.
NF023 binding to XIAP-BIR1: Searching drugs for regulation of the NF-kappa B pathway
Cossu, Federica; Milani, Mario; Grassi, Serena; Malvezzi, Francesca; Corti, Alessandro; Bolognesi, Martino; Mastrangelo, Eloise
Proteins , 83, 2015
DOI: 10.1002/prot.24766
The activator of apoptosis Smac-DIABLO acts as a tetramer in solution
Mastrangelo E.; Vachette P.; Cossu F.; Malvezzi F.; Bolognesi M.; Milani M.
Biophysical journal , 108, 2015
DOI: 10.1016/j.bpj.2014.11.3471
Delivery of Suramin as an Antiviral Agent through Liposomal Systems
Mastrangelo, Eloise; Mazzitelli, Stefania; Fabbri, Jacopo; Rohayem, Jacques; Ruokolainen, Janne; Nykanen, Antti; Milani, Mario; Pezzullo, Margherita; Nastruzzi, Claudio; Bolognesi, Martino
ChemMedChem, 9, 2014
DOI: 10.1002/cmdc.201300563
Naphthalene-sulfonate inhibitors of human norovirus RNA-dependent RNA-polymerase
Tarantino, Delia; Pezzullo, Margherita; Mastrangelo, Eloise; Croci, Romina; Rohayem, Jacques; Robel, Ivonne; Bolognesi, Martino; Milani, Mario
Antiviral research, 102, 2014
DOI: 10.1016/j.antiviral.2013.11.016
PPNDS inhibits murine Norovirus RNA-dependent RNA-polymerase mimicking two RNA stacking bases
Croci, Romina; Tarantino, Delia; Milani, Mario; Pezzullo, Margherita; Rohayem, Jacques; Bolognesi, Martino; Mastrangelo, Eloise
FEBS letters, 588, 2014
DOI: 10.1016/j.febslet.2014.03.021
Structural bases of norovirus RNA dependent RNA polymerase inhibition by novel suramin-related compounds
Croci, Romina; Pezzullo, Margherita; Tarantino, Delia; Milani, Mario; Tsay, Shwuchen; Sureshbabu, Radhakrishnan; Tsai, Yijin; Mastrangelo, Eloise; Rohayem, Jacques; Bolognesi, Martino C.; Hwu, Jihru
PloS one, 9, 2014
DOI: 10.1371/journal.pone.0091765
Evidence for self-association of the alternative sigma factor sigma 54
Sabbatini M, Vezzoli A, Milani M, Bertoni G.
The FEBS journal, 280, 2013
DOI: 10.1111/febs.12129
Is Renalase a Novel Player in Catecholaminergic Signaling? The Mystery of the Catalytic Activity of an Intriguing New Flavoenzyme
Baroni S; Milani M; Pandini V; Pavesi G; Horner D; Aliverti A
Current pharmaceutical design, 19, 2013
Dimeric Smac mimetics/IAP inhibitors as in vivo-active pro-apoptotic agents. Part II: Structural and biological characterization.
Daniele Lecis; Eloise Mastrangelo; Laura Belvisi; Martino Bolognesi; Monica Civera; Federica Cossu; Michelandrea De Cesare; Domenico Delia; Carmelo Drago; Giacomo Manenti; Leonardo Manzoni; Mario Milani; Elisabetta Moroni; Paola Perego; Donatella Potenza;
Bioorganic & medicinal chemistry , 2012
Flaviviral helicase: Insights into the mechanism of action of a motor protein
Mastrangelo E; Bolognesi M; Milani M
Biochemical and biophysical research communications, 417, 2012
DOI: 10.1016/j.bbrc.2011.11.060
Ivermectin is a potent inhibitor of flavivirus replication specifically targeting NS3 helicase activity: new prospects for an old drug
Mastrangelo E; Pezzullo M; De Burghgraeve T; Kaptein S; Pastorino B; Dallmeier K; de Lamballerie X; Neyts J; Hanson AM; Frick DN; Bolognesi M; Milani M
Journal of antimicrobial chemotherapy, 67, 2012
Structural Insight into Inhibitor of Apoptosis Proteins Recognition by a Potent Divalent Smac-Mimetic
Cossu F; Milani M; Vachette P; Malvezzi F; Grassi S; Lecis D; Delia D; Drago C; Seneci P; Bolognesi M; Mastrangelo E
PloS one, 7, 2012
DOI: 10.1371/journal.pone.0049527
Structure-Based Inhibition of Norovirus RNA-Dependent RNA Polymerases
Mastrangelo E; Pezzullo M; Tarantino D; Petazzi R; Germani F; Kramer D; Robel I; Rohayem J; Bolognesi M; Milani M
Journal of Molecular Biology, 419, 2012
FAD-binding site and NADP reactivity in human renalase: a new enzyme involved in blood pressure regulation
Milani M, Ciriello F, Baroni S, Pandini V, Canevari G, Bolognesi M, Aliverti A
Journal of molecular biology, 411, 2011
Recognition of Smac-mimetic compounds by the BIR domain of cIAP1
Cossu F, Malvezzi F, Canevari G, Mastrangelo E, Lecis D, Delia D, Seneci P, Scolastico C, Bolognesi M, Milani M
Protein science, 19, 2010
Hemoprotein time-resolved X-ray crystallography
Milani, M; Nardini, M; Pesce, A; Mastrangelo, E; Bolognesi, M
IUBMB LIFE (PRINT), 60, 2008
DOI: 10.1002/iub.23
Ligand binding to truncated hemoglobin N from Mycobacterium tuberculosis is strongly modulated by the interplay between the distal heme pocket residues and internal water
Ouellet, Yh; Daigle, R; Lague, P; Dantsker, D; Milani, M; Bolognesi, M; Friedman, Jm; Guertin, M
Mapping heme-ligand tunnels in group I truncated(2/2) hemoglobins
Pesce, A; Milani, M; Nardini, M; Bolognesi, M
Structural determinants of ligand migration in Mycobacterium tuberculosis truncated hemoglobin O
Boechi, L; Marti, Ma; Milani, M; Bolognesi, M; Luque, Fj; Estrin, Da
Ferredoxin-NADP(+) reductase from Plasmodium falciparum undergoes NADP(+)-dependent dimerization and inactivation: Functional and crystallographic analysis
Milani M; Balconi E; Aliverti A; Mastrangelo E; Seeber F; Bolognesi M; Zanetti G
JOURNAL OF MOLECULAR BIOLOGY, 367, 2007
DOI: 10.1016/j.jmb.2007.01.005
Mycobacterial truncated hemoglobins: From genes to functions
Ascenzi, P; Bolognesi, M; Milani, M; Guertin, M; Visca, P
GENE, 398, 2007
DOI: 10.1016/j.gene.2007.02.043
Protein fold and structure in the truncated (2/2) globin family
Nardini, M; Pesce, A; Milani, M; Bolognesi, M
GENE, 398, 2007
DOI: 10.1016/j.gene.2007.02.045
The roles of Tyr(CD1) and Trp(G8) in Mycobacterium tuberculosis truncated hemoglobin O in ligand binding and on the heme distal site architectures
Ouellet, H; Milani, M; LaBarre, M; Bolognesi, M; Couture, M; Guertin, M
BIOCHEMISTRY (EASTON), 46, 2007
DOI: 10.1021/bi7010288
Ligand interactions in the distal heme pocket of Mycobacterium tuberculosis truncated hemoglobin N: Roles of TyrB10 and GlnE11 residues
Ouellet, Y; Milani, M; Couture, M; Bolognesi, M; Guertin, M
BIOCHEMISTRY (EASTON), 45, 2006
DOI: 10.1021/bi060112o
Ligand-induced dynamical regulation of NO conversion in Mycobacterium tuberculosis truncated hemoglobin-N
Bidonchanal, A; Marti, Ma; Crespo, A; Milani, M; Orozco, M; Bolognesi, M; Luque, Fj; Estrin, Da
PROTEINS (ONLINE), 64, 2006
DOI: 10.1002/prot.21004
Nitric oxide scavenging by Mycobacterium leprae GlbO involves the formation of the ferric heme-bound peroxynitrite intermediate
Ascenzi, P; Bocedi, A; Bolognesi, M; Fabozzi, G; Milani, M; Visca, P
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (PRINT), 339, 2006
DOI: 10.1016/j.bbrc.2005.11.031
Preliminary characterization of (nucleoside-2'-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses.
Mastrangelo, E; Bollati, M; Milani, M; De Lamballerie, X; Brisbarre, N; Dalle, K; Lantez, V; Egloff, MP; Coutard, B; Canard, B; Gould, E; Forrester, N; Bolognesi, M
ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 63, 2006
Preliminary crystallographic characterization of an RNA helicase from Kunjin virus
Mastrangelo, E; Bollati, M; Milani, M; Brisbarre, N; de Lamballerie, X; Coutard, B; Canard, B; Khromykh, A; Bolognesi, M
ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 62, 2006
Mario Milani
Research staff
Since 2005 I am a CNR researcher. I work on the relationship between structure and function of proteins involved in different human diseases, using various experimental and computational techniques. In particular, I work in expression and purification of recombinant proteins, biophysical and biochemical characterization of purified proteins, production of protein crystals, structural analysis with X-ray crystallography, SAXS and electron microscopy. In addition, I work in in silico docking for the identification of inhibitors to be developed as new drugs and in molecular dynamics simulations.
1998-2002, PhD in Physics at the University of Genoa.
1997-1998, Experimental thesis in Physics, University of Rome "La Sapienza". Vote: 110/110
orcid://0000-0001-6098-3991,
Contact:
- Email mario.milani@ibf.cnr.it
- Phone +39-02-23699540
- Location Milano
Website:
http://users.unimi.it/biolstru/Home.html
Research interests:
- Structural analysis of proteins involved in various human diseases and research of new therapeutic strategies (Principal investigator)
- Towards deciphering ribosome heterogeneity and polyribosome code to understand translational control of gene expression
- Type I BIR inhibitors for the regulation of NF-kB pathway
- Therapeutic effects of retromer stabilization in Amyotrophic Lateral Sclerosis
- Yeaz, the resuscitation-promoting factor
- Structural and biochemical bases of the pathogenicity of the Apoptosis Inducing Factor (AIF)
- Translational defects in motor neuron diseases
- Structure-based drug discovery on viral proteins
- Gelsolin physiopathology
- Structure-based drug discovery
Further collaborations:
- Pierfausto Seneci (Università degli studi di Milano)
- Alessandro Aliverti (Università degli studi di Milano)
- Giovanni Bertoni (Università degli studi di Milano)
- Alessandro Marcello (ICGEB, Trieste)
- Oscar Burrone (ICGEB, Trieste)
- Francesca Arnoldi (ICGEB, Trieste)
- Violetta Cecchetti (Università degli studi di Perugia)
- Giuseppe Manfroni (Università degli studi di Perugia)
- Daniele Lecis (Istituto Tumori Milano)
- Daniele dell’Orco (Università degli studi di Verona)
- Leonardo Manzoni (Cnr-Istm)
- Robert Kleta (UCL)
Financial support:
- Telethon 2017-2020: Cone dystrophies and retinal degeneration from protein structures to biological networks. Toward the design of therapeutic molecules. Grant number: GGP16010. Ruolo: partner coordinator.
- Grant Instruct 2018-2019 grant Instruct (https://www.structuralbiology.eu/) "Structural analysis of the interaction between retinal guanylyl cyclase 1 (GC1) and guanylate cyclase activating protein 1 (GCAP1)". Ruolo: PI.
Key publications:
Reichold M, Klootwijk ED, Reinders J, Otto EA, Milani M, Broeker C, et al. (2017) Glycine amidinotransferase (GATM), renal Fanconi syndrome, and kidney failure. J Am Soc Nephrol. 29(7):1849-1858
Tarantino D, Cannalire R, Mastrangelo E, Croci R, Querat G, Barreca ML, Bolognesi M, Manfroni G, Cecchetti V, Milani M (2016) Targeting flavivirus RNA dependent RNA polymerase through a pyridobenzothiazole inhibitor. Antiviral research 134:226-235
Mastrangelo E, Vachette P, Cossu F, Malvezzi F, Bolognesi M, Milani M (2015) The activator of apoptosis Smac-DIABLO acts as a tetramer in solution. Biophysical journal 108:714-723
Tarantino D, Pezzullo M, Mastrangelo E, Croci R, Rohayem J, Robel I, Bolognesi M, Milani M (2014) Naphthalene-sulfonate inhibitors of human norovirus RNA-dependent RNA-polymerase. Antiviral research 102:23-28
Mastrangelo E, Pezzullo M, De Burghgraeve T, Kaptein S, Pastorino B, Dallmeier K, de Lamballerie X, Neyts J, Hanson AM, Frick DN, Bolognesi M, Milani M (2012) Ivermectin is a potent inhibitor of flavivirus replication specifically targeting NS3 helicase activity: new prospects for an old drug. Journal of Antimicrobial Chemotherapy 67(8):1884-1894
Publications (61):
Structural snapshots of the aldol condensation reaction of the enzyme trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from Pseudomonas fluorescens N3Ferrara, Silvia; Braggiotti, Bianca; Mastrangelo, Eloise; Di Gennaro, Patrizia; Bertoni, Giovanni; Milani, Mario
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 747, 2025
DOI: 10.1016/j.bbrc.2024.151281
4-(3-Phenylsulfonylindol-2-yl)-1-(pyridin-2-yl)piperazinyl-methanones as Potent Inhibitors of both SARS-CoV-2 and HCoV-OC43 Viruses
Puxeddu, M.; Donalisio, M.; Bugert, J. J.; Corona, A.; Cocomazzi, P.; MILANI DE MAYO DE MARI, Mario; Hucke, F.; Arduino, I.; Esposito, F.; Moretti, P.; Ortore, M. G.; Nalli, M.; Manetto, S.; Mazzoccanti, G.; Bigogno, C.; Dondio, G.; Scio, P.; Coluccia, A.; Fracella, M.; Antonelli, G.; Lembo, D.; Tramontano, E.; Silvestri, R.; Mastrangelo, E.; La Regina, G.
ACS INFECTIOUS DISEASES, 10, 2024
DOI: 10.1021/acsinfecdis.4c00108
CHCHD4 binding affects the active site of apoptosis inducing factor (AIF): Structural determinants for allosteric regulation
Fagnani, E.; Cocomazzi, P.; Pellegrino, S.; Tedeschi, G.; Scalvini, F. G.; Cossu, F.; Da Vela, S.; Aliverti, A.; Mastrangelo, E.; Milani, M.
STRUCTURE, 2024
DOI: 10.1016/j.str.2024.02.008
Stabilization of the retromer complex: Analysis of novel binding sites of bis-1,3-phenyl guanylhydrazone 2a to the VPS29/VPS35 interface
Fagnani, E.; Bonì, F.; Seneci, P.; Gornati, D.; Muzio, L.; Mastrangelo, E.; MILANI DE MAYO DE MARI, Mario
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 23, 2024
DOI: 10.1016/j.csbj.2024.02.026
Studying an orphan disease by a biophysical approach: The case of cblC
Vilasi, S.; Longo, L.; Randazzo, L.; Bollati, M.; Carrotta, R.; Costa, M. A.; De Rosa, M.; Mangione, M. R.; Martorana, V.; Milani, M.; Ortore, M. G.; Tutone, M.; Passantino, R.
IL NUOVO CIMENTO C, 47, 2024
DOI: 10.1393/ncc/i2024-24318-2
Virucidal Activity of the Pyridobenzothiazolone Derivative HeE1-17Y against Enveloped RNA Viruses
Bonotto R.M.; Boni F.; Milani M.; Chaves-Sanjuan A.; Franze S.; Selmin F.; Felicetti T.; Bolognesi M.; Konstantinidou S.; Poggianella M.; Marquez C.L.; Dattola F.; Zoppe M.; Manfroni G.; Mastrangelo E.; Marcello A.
viruses, 14, 2022
DOI: 10.3390/v14061157
A novel hotspot of gelsolin instability triggers an alternative mechanism of amyloid aggregation
Bollati, Michela; Diomede, Luisa; Giorgino, Toni; Natale, Carmina; Fagnani, Elisa; Boniardi, Irene; Barbiroli, Alberto; Alemani, Rebecca; Beeg, Marten; Gobbi, Marco; Fakin, Ana; Mastrangelo, Eloise; Milani, Mario; Presciuttini, Gianluca; Gabellieri, Edi; Cioni, Patrizia; de Rosa, Matteo
computational and structural biotechnology journalissn: 2001-0370chalmers university of technology,, 19, 2021
DOI: 10.1016/j.csbj.2021.11.025
Combined in silico and in vitro approaches identified the antipsychotic drug lurasidone and the antiviral drug elbasvir as SARS-CoV2 and HCoV-OC43 inhibitors
Milani M.; Donalisio M.; Bonotto R.M.; Schneider E.; Arduino I.; Boni F.; Lembo D.; Marcello A.; Mastrangelo E.
antiviral research (print)issn: 0166-3542elsevier/north-holland,, 189, 2021
DOI: 10.1016/j.antiviral.2021.105055
Structure-based identification of a new IAP-targeting compound that induces cancer cell death inducing NF-kappa B pathway
Cossu, Federica; Camelliti, Simone; Lecis, Daniele; Sorrentino, Luca; Majorini, Maria Teresa; Milani, Mario; Mastrangelo, Eloise
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, 19, 2021
DOI: 10.1016/j.csbj.2021.11.034
Synthesis and Characterization of Novel Mono- And Bis-Guanyl Hydrazones as Potent and Selective ASIC1 Inhibitors Able to Reduce Brain Ischemic Insult
Gornati D.; Ciccone R.; Vinciguerra A.; Ippati S.; Pannaccione A.; Petrozziello T.; Pizzi E.; Hassan A.; Colombo E.; Barbini S.; Milani M.; Caccavone C.; Randazzo P.; Muzio L.; Annunziato L.; Menegon A.; Secondo A.; Mastrangelo E.; Pignataro G.; Seneci P.
journal of medicinal chemistry (online), 64, 2021
DOI: 10.1021/acs.jmedchem.1c00305
Computational and Experimental Characterization of NF023, A Candidate Anticancer Compound Inhibiting cIAP2/TRAF2 Assembly
Federica Cossu, Luca Sorrentino, Elisa Fagnani, Mattia Zaffaroni, Mario Milani, Toni Giorgino, Eloise Mastrangelo
Journal of chemical information and modeling, 60, 2020
DOI: 10.1021/acs.jcim.0c00518
Missense mutations affecting Ca2+-coordination in GCAP1 lead to cone-rod dystrophies by altering protein structural and functional properties
Dal Cortivo, Giuditta; Marino, Valerio; Boni, Francesco; Milani, Mario; Dell'Orco, Daniele
Biochimica Et Biophysica Acta-molecular Cell Research, 1867, 2020
DOI: 10.1016/j.bbamcr.2020.118794
Modulation of guanylate cyclase activating protein 1 (Gcap1) dimeric assembly by ca2+ or mg2+: Hints to understand protein activity
Bonì, F., Marino, V., Bidoia, C., Mastrangelo, E., Barbiroli, A., Dell’Orco, D., Milani, M.
Biomolecules, 10, 2020
DOI: 10.3390/biom10101408
Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis
Muzio, Luca; Sirtori, Riccardo; Gornati, Davide; Eleuteri, Simona; Fossaghi, Andrea; Brancaccio, Diego; Manzoni, Leonardo; Ottoboni, Linda; De Feo, Luca; Quattrini, Angelo; Mastrangelo, Eloise; Sorrentino, Luca; Scalone, Emanuele; Comi, Giancarlo; Marinel
Nature Communications, 11, 2020
DOI: 10.1038/s41467-020-17524-7
The structure of N184K amyloidogenic variant of gelsolin highlights the role of the H-bond network for protein stability and aggregation properties
Matteo de Rosa, Alberto Barbiroli, Francesco Bonì, Emanuele Scalone, Davide Mattioni, Maria A Vanoni, Marco Patrone, Michela Bollati, Eloise Mastrangelo, Toni Giorgino, Mario Milani
European biophysics journal, 49, 2020
DOI: 10.1007/s00249-019-01409-9
An alternative non-proteolytic mechanism may underlie AGel amyloidosis
Bonì F, Milani M, Mastrangelo E, Babiroli A, Diomede L, de Rosa M
Amyloid: the international journal of experimental and clinical investigation: the official journal of the International Society of Amyloidosis, 26, 2019
DOI: 10.1080/13506129.2019.1582510
Broad spectrum anti-flavivirus pyridobenzothiazolones leading to less infective virions
Cannalire R, Tarantino D, Piorkowski G, Carletti T, Massari S, Felicetti T, Barreca ML, Sabatini S, Tabarrini O, Marcello A, Milani M, Cecchetti V, Mastrangelo E, Manfroni G, Querat G
Antiviral research, 167, 2019
DOI: 10.1016/j.antiviral.2019.03.004
High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion
Bollati M, Scalone E, Bonì F, Mastrangelo E, Giorgino T, Milani M, de Rosa M
Biochemical and biophysical research communications, 518, 2019
DOI: 10.1016/j.bbrc.2019.08.013
Nanobody interaction unveils structure, dynamics and proteotoxicity of the Finnish-type amyloidogenic gelsolin variant
Giorgino T, Mattioni D, Hassan A, Milani M, Mastrangelo E, Barbiroli A, Verhelle A, Gettemans J, Barzago MM, Diomede L, de Rosa M
Biochimica et biophysica acta. Molecular basis of disease, 1865, 2019
DOI: 10.1016/j.bbadis.2019.01.010
Structural and functional characterization of TgpA, a critical protein for the viability of Pseudomonas aeruginosa
Uruburu M, Mastrangelo E, Bolognesi M, Ferrara S, Bertoni G, Milani M
Journal of structural biology, 205, 2019
DOI: 10.1016/j.jsb.2018.12.004
Structure-Activity Relationship of NF023 Derivatives Binding to XIAP-BIR1
Sorrentino L, Cossu F, Milani M, Malkoc B, Huang WC, Tsay SC, Ru Hwu J, Mastrangelo E
ChemistryOpen, 8, 2019
DOI: 10.1002/open.201900059
Targeting the BIR Domains of Inhibitor of Apoptosis (IAP) Proteins in Cancer Treatment
Cossu F, Milani M, Mastrangelo E, Lecis D
Computational and Structural Biotechnology Journal, 17, 2019
DOI: 10.1016/j.csbj.2019.01.009
The structure of N184K amyloidogenic variant of gelsolin highlights the role of the H-bond network for protein stability and aggregation properties
de Rosa M, Barbiroli A, Bonì F, Scalone E, Mattioni D, Vanoni MA, Patrone M, Bollati M, Mastrangelo E, Giorgino T, Milani M
European biophysics journal, 49, 2019
DOI: 10.1007/s00249-019-01409-9
A stereospecific carboxyl esterase from Bacillus coagulans hosting nonlipase activity within a lipase-like fold
De Vitis V, Nakhnoukh, Pinto A, Contente ML, Barbiroli A, Milani M, Bolognesi M, Molinari F, Gourlay LJ, Romano D
The FEBS journal (online), 285, 2018
DOI: 10.1111/febs.14368
Functionalized 2,1-benzothiazine 2,2-dioxides as new inhibitors of Dengue NS5 RNA-dependent RNA polymerase
Cannalire R, Tarantino D, Astolfi A, Barreca ML, Sabatini S, Massari S, Tabarrini O, Milani M, Querat G, Mastrangelo E, Manfroni G, Cecchetti V
European journal of medicinal chemistry (online), 143, 2018
DOI: 10.1016/j.ejmech.2017.10.064
Gelsolin pathogenic Gly167Arg mutation promotes domain-swap dimerization of the protein
Bonì F, Milani M, Barbiroli A, Diomede L, Mastrangelo E, de Rosa M
Human molecular genetics (online), 27, 2018
DOI: 10.1093/hmg/ddx383
Glycine Amidinotransferase (GATM), Renal Fanconi Syndrome, and Kidney Failure
Reichold M, Klootwijk ED, Reinders J, Otto EA, Milani M, Broeker C, Laing C, Wiesner J, Devi S, Zhou W, Schmitt R, Tegtmeier I, Sterner C, Doellerer H, Renner K, Oefner PJ, Dettmer K, Simbuerger JM, Witzgall R, Stanescu HC, Dumitriu S, Iancu D, Patel V, M
Journal of the American Society of Nephrology, 29, 2018
DOI: 10.1681/ASN.2017111179
Identification of a small molecule that compromises the structural integrity of viroplasms and rotavirus double-layered particles
Eichwald C, De Lorenzo G, Schraner EM, Papa G, Bollati M, Swuec P, de Rosa M, Milani M, Mastrangelo E, Ackermann M, Burrone OR, Arnoldi F
Journal of virology, 92, 2018
DOI: 10.1128/JVI.01943-17
Role and inhibition of GLI1 protein in cancer
Mastrangelo E, Milani M
Lung cancer , 9, 2018
DOI: 10.2147/LCTT.S124483
Structure-based design and molecular profiling of Smac-mimetics selective for cellular IAPs
Corti A, Milani M, Lecis D, Seneci P, de Rosa M, Mastrangelo E, Cossu F
The FEBS journal, 285, 2018
DOI: 10.1111/febs.14616
Structural bases of the altered catalytic properties of a pathogenic variant of apoptosis inducing factor
Sorrentino L, Cossu F, Milani M, Aliverti A, Mastrangelo E
Biochemical and biophysical research communications, 490, 2017
DOI: 10.1016/j.bbrc.2017.06.156
Crystal structure of YeaZ from Pseudomonas aeruginosa
Vecchietti D, Ferrara S, Rusmini R, Macchi R, Milani M, Bertoni G
Biochemical and biophysical research communications, 470, 2016
DOI: 10.1016/j.bbrc.2016.01.008
Molecular basis of a novel renal amyloidosis due to N184K gelsolin variant
Bonì F, Milani M, Porcari R, Barbiroli A, Ricagno S, de Rosa M
Scientific reports , 6, 2016
DOI: 10.1038/srep33463.
NF023 binding to XIAP-BIR1: Searching drugs for regulation of the NF-kappa B pathway
Cossu, Federica; Milani, Mario; Grassi, Serena; Malvezzi, Francesca; Corti, Alessandro; Bolognesi, Martino; Mastrangelo, Eloise
Proteins , 83, 2015
DOI: 10.1002/prot.24766
The activator of apoptosis Smac-DIABLO acts as a tetramer in solution
Mastrangelo E.; Vachette P.; Cossu F.; Malvezzi F.; Bolognesi M.; Milani M.
Biophysical journal , 108, 2015
DOI: 10.1016/j.bpj.2014.11.3471
Delivery of Suramin as an Antiviral Agent through Liposomal Systems
Mastrangelo, Eloise; Mazzitelli, Stefania; Fabbri, Jacopo; Rohayem, Jacques; Ruokolainen, Janne; Nykanen, Antti; Milani, Mario; Pezzullo, Margherita; Nastruzzi, Claudio; Bolognesi, Martino
ChemMedChem, 9, 2014
DOI: 10.1002/cmdc.201300563
Naphthalene-sulfonate inhibitors of human norovirus RNA-dependent RNA-polymerase
Tarantino, Delia; Pezzullo, Margherita; Mastrangelo, Eloise; Croci, Romina; Rohayem, Jacques; Robel, Ivonne; Bolognesi, Martino; Milani, Mario
Antiviral research, 102, 2014
DOI: 10.1016/j.antiviral.2013.11.016
PPNDS inhibits murine Norovirus RNA-dependent RNA-polymerase mimicking two RNA stacking bases
Croci, Romina; Tarantino, Delia; Milani, Mario; Pezzullo, Margherita; Rohayem, Jacques; Bolognesi, Martino; Mastrangelo, Eloise
FEBS letters, 588, 2014
DOI: 10.1016/j.febslet.2014.03.021
Structural bases of norovirus RNA dependent RNA polymerase inhibition by novel suramin-related compounds
Croci, Romina; Pezzullo, Margherita; Tarantino, Delia; Milani, Mario; Tsay, Shwuchen; Sureshbabu, Radhakrishnan; Tsai, Yijin; Mastrangelo, Eloise; Rohayem, Jacques; Bolognesi, Martino C.; Hwu, Jihru
PloS one, 9, 2014
DOI: 10.1371/journal.pone.0091765
Evidence for self-association of the alternative sigma factor sigma 54
Sabbatini M, Vezzoli A, Milani M, Bertoni G.
The FEBS journal, 280, 2013
DOI: 10.1111/febs.12129
Is Renalase a Novel Player in Catecholaminergic Signaling? The Mystery of the Catalytic Activity of an Intriguing New Flavoenzyme
Baroni S; Milani M; Pandini V; Pavesi G; Horner D; Aliverti A
Current pharmaceutical design, 19, 2013
Dimeric Smac mimetics/IAP inhibitors as in vivo-active pro-apoptotic agents. Part II: Structural and biological characterization.
Daniele Lecis; Eloise Mastrangelo; Laura Belvisi; Martino Bolognesi; Monica Civera; Federica Cossu; Michelandrea De Cesare; Domenico Delia; Carmelo Drago; Giacomo Manenti; Leonardo Manzoni; Mario Milani; Elisabetta Moroni; Paola Perego; Donatella Potenza;
Bioorganic & medicinal chemistry , 2012
Flaviviral helicase: Insights into the mechanism of action of a motor protein
Mastrangelo E; Bolognesi M; Milani M
Biochemical and biophysical research communications, 417, 2012
DOI: 10.1016/j.bbrc.2011.11.060
Ivermectin is a potent inhibitor of flavivirus replication specifically targeting NS3 helicase activity: new prospects for an old drug
Mastrangelo E; Pezzullo M; De Burghgraeve T; Kaptein S; Pastorino B; Dallmeier K; de Lamballerie X; Neyts J; Hanson AM; Frick DN; Bolognesi M; Milani M
Journal of antimicrobial chemotherapy, 67, 2012
Structural Insight into Inhibitor of Apoptosis Proteins Recognition by a Potent Divalent Smac-Mimetic
Cossu F; Milani M; Vachette P; Malvezzi F; Grassi S; Lecis D; Delia D; Drago C; Seneci P; Bolognesi M; Mastrangelo E
PloS one, 7, 2012
DOI: 10.1371/journal.pone.0049527
Structure-Based Inhibition of Norovirus RNA-Dependent RNA Polymerases
Mastrangelo E; Pezzullo M; Tarantino D; Petazzi R; Germani F; Kramer D; Robel I; Rohayem J; Bolognesi M; Milani M
Journal of Molecular Biology, 419, 2012
FAD-binding site and NADP reactivity in human renalase: a new enzyme involved in blood pressure regulation
Milani M, Ciriello F, Baroni S, Pandini V, Canevari G, Bolognesi M, Aliverti A
Journal of molecular biology, 411, 2011
Recognition of Smac-mimetic compounds by the BIR domain of cIAP1
Cossu F, Malvezzi F, Canevari G, Mastrangelo E, Lecis D, Delia D, Seneci P, Scolastico C, Bolognesi M, Milani M
Protein science, 19, 2010
Hemoprotein time-resolved X-ray crystallography
Milani, M; Nardini, M; Pesce, A; Mastrangelo, E; Bolognesi, M
IUBMB LIFE (PRINT), 60, 2008
DOI: 10.1002/iub.23
Ligand binding to truncated hemoglobin N from Mycobacterium tuberculosis is strongly modulated by the interplay between the distal heme pocket residues and internal water
Ouellet, Yh; Daigle, R; Lague, P; Dantsker, D; Milani, M; Bolognesi, M; Friedman, Jm; Guertin, M
Mapping heme-ligand tunnels in group I truncated(2/2) hemoglobins
Pesce, A; Milani, M; Nardini, M; Bolognesi, M
Structural determinants of ligand migration in Mycobacterium tuberculosis truncated hemoglobin O
Boechi, L; Marti, Ma; Milani, M; Bolognesi, M; Luque, Fj; Estrin, Da
Ferredoxin-NADP(+) reductase from Plasmodium falciparum undergoes NADP(+)-dependent dimerization and inactivation: Functional and crystallographic analysis
Milani M; Balconi E; Aliverti A; Mastrangelo E; Seeber F; Bolognesi M; Zanetti G
JOURNAL OF MOLECULAR BIOLOGY, 367, 2007
DOI: 10.1016/j.jmb.2007.01.005
Mycobacterial truncated hemoglobins: From genes to functions
Ascenzi, P; Bolognesi, M; Milani, M; Guertin, M; Visca, P
GENE, 398, 2007
DOI: 10.1016/j.gene.2007.02.043
Protein fold and structure in the truncated (2/2) globin family
Nardini, M; Pesce, A; Milani, M; Bolognesi, M
GENE, 398, 2007
DOI: 10.1016/j.gene.2007.02.045
The roles of Tyr(CD1) and Trp(G8) in Mycobacterium tuberculosis truncated hemoglobin O in ligand binding and on the heme distal site architectures
Ouellet, H; Milani, M; LaBarre, M; Bolognesi, M; Couture, M; Guertin, M
BIOCHEMISTRY (EASTON), 46, 2007
DOI: 10.1021/bi7010288
Ligand interactions in the distal heme pocket of Mycobacterium tuberculosis truncated hemoglobin N: Roles of TyrB10 and GlnE11 residues
Ouellet, Y; Milani, M; Couture, M; Bolognesi, M; Guertin, M
BIOCHEMISTRY (EASTON), 45, 2006
DOI: 10.1021/bi060112o
Ligand-induced dynamical regulation of NO conversion in Mycobacterium tuberculosis truncated hemoglobin-N
Bidonchanal, A; Marti, Ma; Crespo, A; Milani, M; Orozco, M; Bolognesi, M; Luque, Fj; Estrin, Da
PROTEINS (ONLINE), 64, 2006
DOI: 10.1002/prot.21004
Nitric oxide scavenging by Mycobacterium leprae GlbO involves the formation of the ferric heme-bound peroxynitrite intermediate
Ascenzi, P; Bocedi, A; Bolognesi, M; Fabozzi, G; Milani, M; Visca, P
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (PRINT), 339, 2006
DOI: 10.1016/j.bbrc.2005.11.031
Preliminary characterization of (nucleoside-2'-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses.
Mastrangelo, E; Bollati, M; Milani, M; De Lamballerie, X; Brisbarre, N; Dalle, K; Lantez, V; Egloff, MP; Coutard, B; Canard, B; Gould, E; Forrester, N; Bolognesi, M
ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 63, 2006
Preliminary crystallographic characterization of an RNA helicase from Kunjin virus
Mastrangelo, E; Bollati, M; Milani, M; Brisbarre, N; de Lamballerie, X; Coutard, B; Canard, B; Khromykh, A; Bolognesi, M
ACTA CRYSTALLOGRAPHICA. SECTION F, STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 62, 2006